The BIACORE 2000 by Biacore AB is a biomolecular analyzer that utilizes surface plasmon resonance (SPR) technology to monitor biomolecular interactions in real time. SPR is a noninvasive optical measuring technique that does not require the labelling of interacting components. It is used to find the mass concentration of biomolecules that are adjacent to a specially prepared surface. Because the response is mostly independent of biomolecular nature, the same detection technique can be used across all investigations. The real time biomolecular interaction analysis (BIA) can detect surface protein concentrations as low as a few pg/mm2 and can similarly measure differences in surface concentration as quick as a tenth of a second. In this way, the platform is able to determine binding specificity, kinetics, and concentration for analytes as small as 150 daltons and as large as 106 daltons, making it incredibly flexible in application. Integrated fluidics and flow through technology provide maximum reproducibility in sample handling and makes kinetic calculations practical and intuitive.